Dorsey is giving up the stock, currently worth more than USD 200 million, so Twitter can award the shares to its employees, according to documents filed today with the Securities and Exchange Commission.
The gesture comes a week after Dorsey laid off 8 per cent of Twitter's workforce in an effort to make the company profitable for the first time in its nine-year history.
"I'd rather have a smaller part of something big than a bigger part of something small," Dorsey tweeted about surrendering some of his stock.
"I'm confident we can make Twitter big!"
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Twitter said Dorsey's shares will be earmarked for stock issued to employees next year under the company's incentive plan.
Dorsey will still own 15 million Twitter shares worth about USD 460 million, based on Twitter's current market value.
Twitter's stock gained USD 1.51, or more than 5 per cent, to USD 30.66 in today's afternoon trading.
Twitter Inc. Brought back Dorsey, one of its co-founders, as its CEO in hopes that he can figure out a way to make the messaging service more appealing beyond its core audience of about 300 million users.
By comparison, Facebook's social networking service has 1.5 billion users, even though it is only two years older than Twitter.
Dorsey was Twitter's original CEO, but was ousted because the company's board didn't think he was the right leader at that time.
Dorsey has given back nearly 15.1 million shares of Square stock during the past two years, too, according to Square's IPO documents.
He remains by far Square's largest shareholder with a 24 per cent stake in that San Francisco company, which is located a block away from Twitter's headquarters.