"We see a significant opportunity to increase growth as we continue to improve the core service," chief executive Jack Dorsey said while releasing quarterly results showing more losses.
"We have a clear plan, and we're making the necessary changes to ensure Twitter is positioned for long-term growth."
Twitter reported a net loss for the quarter of $103 million, compared with a USD 132 million loss a year earlier. Revenues meanwhile grew eight percent to $616 million, most of that from advertising.
Twitter said the restructuring "is intended to create greater focus and efficiency" and help move toward profitability in 2017.
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Twitter was widely reported to be in talks to sell the one-to-many messaging service, and it held meetings with Google parent Alphabet and cloud computing giant Salesforce. But no deal materialized and Salesforce said Twitter was not a good fit for the group.
"The key drivers of future revenue growth are trending positive, and we remain confident in Twitter's future," he said.
The cuts would amount to about 350 jobs based on the Twitter website's headcount of 3,860 employees worldwide. Twitter will incur a charge of USD 10 million to USD 20 million for the reorganization.
Twitter shares jumped more than five percent in pre-market trades, after the results topped most analyst forecasts and after one financial blog reported that Walt Disney Co. Had reopened talks on an acquisition.
Twitter's 2013 public offering was among the most anticipated in the sector, but after a brief rise, the shares have been slumping. Based on its most recent share price, Twitter's market value is some $12 billion.
Dorsey, one of the co-founders of the messaging platform, returned as CEO last year as part of an effort to revive growth.
Under Dorsey, Twitter added new services such as live video including partnerships with major sports organizations and new advertising options. Twitter has drawn large audiences for events such as the US presidential debates and NFL football games.
Analysts remain cautious about Twitter's ability to break out of its rut and accelerate growth. It is far behind Facebook, which has an audience of over 1.7 billion, and Facebook-owned Instagram, with some 500 million.
The research firm eMarketer estimates Twitter's share of US social network users will decline to 28.1 percent this year and will continue to drop through 2020, as it loses users to Snapchat, Instagram and messaging apps.
According to eMarketer, around 16 percent of the US population uses Twitter compared to 51 percent for Facebook. On a worldwide basis, Twitter penetration is even lower at only 3.9 per cent, according to the research group.