The incident took place when the victims were returning home from Mandli region after taking part in a condolence meeting.
"Their bike collided with a tractor-trolley, which led to their death on the spot," Meghnagar Police Station In-charge M L Bhabar said.
The deceased have been identified as Mesu Medha (50) and Gulla Vasunia (48), he said adding they belong to Meghnagar town of the district, the official said.
A case has been registered in this regard and further investigations are on.