The discovery brings the number of recognised mouse lemur species to 20, making them the most diverse group of lemurs known. Since these shy, nocturnal primates look so much alike, it's only possible to tell them apart with genetic sequencing.
The new mouse lemurs weigh about 65 to 85 grams and have grey-brown fur.
"You can't really tell them apart just looking at them through binoculars in the rainforest," said senior author Peter Kappeler of the German Primate Center in Goettingen, who earned his PhD at Duke University.
The researchers named the other new species the Marohita mouse lemur, or Microcebus marohita, after the forest where it was found. In Malagasy, the word "marohita" means "many views."
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"Despite its species' name, this mouse lemur is threatened by ongoing habitat destruction, and 'many views' of its members are unlikely," the researchers wrote in International Journal of Primatology.
The two new species were first captured by co-author Rodin Rasoloarison of the University of Antananarivo in Madagascar during trips to the eastern part of the country in 2003 and 2007. Rasoloarison weighed and measured them and took tiny skin samples for genetic analysis in the lab.
Their genetic analyses were published in 2010, but this is the first time the species have been formally named and described.
During a 2012 return trip to the forest where the Marohita mouse lemur lives, Rasoloarison discovered that much of the lemur's forest home had been cleared since his first visit in 2003.
The state of the lemur's habitat prompted the International Union for the Conservation of Nature (IUCN) to classify the new species as "endangered" even before it was formally described.
Mouse lemurs have lived in Madagascar for 7 to 10 million years. But since humans arrived on the island some 2,500 years ago, logging and slash and burn agriculture have taken their toll on the forests where these tree-dwelling primates live.