The horses had large open wounds and very poor body condition, including protruding rib cages and pelvic bones, a release by the People for the Ethical Treatment of Animals (PETA) said today.
The carriages, known as Victoria, were plying in the restricted areas, it said.
The operation was conducted on Saturday with the support of an expert team authorised by the Animal Welfare Board of India (AWBI) and consisting of a veterinarian from PETA and an animal-welfare officer from NGO, Animal Rahat, it said.
Earlier, a week ago, police had seized three carriage horses.
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Some carriage drivers threatened the team members during inspection of horses despite having authorisation from the AWBI and an order from Ashok Dudhe, Deputy Commissioner of Police (Operations), PETA said.
Police has registered two non-cognisable offence complaints against the carriage owners and drivers under Prevention of Cruelty to Animals Act, 1960.
The seized horses were sent to the Bombay Society for Prevention of Cruelty to Animals hospital in Parel.