Didi, China's ride-hailing leader, and mobile giant SoftBank will invest up to USD 2.0 billion while USD 500 million is expected to come from other new and existing investors.
Grab said it amounts to the largest single financing in Southeast Asia. Both Didi and SoftBank are already investors in Grab.
The Singapore-based firm said it would use the fresh funds to bolster its leading position in the ride-hailing industry and invest in a mobile payment platform called GrabPay.
"With their support, Grab will achieve an unassailable market lead in ridesharing, and build on this to make GrabPay the payment solution of choice for Southeast Asia."
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Grab said it has a market share of 95 per cent in third- party taxi-hailing and 71 per cent in private-vehicle hailing in Southeast Asia, far ahead of main rival Uber.
Grab offers private car, taxi, motorbike and carpooling services in seven countries and 65 cities across Southeast Asia, a region of 650 million people.
"Both companies look forward to working together with communities and policymakers across Asia to fully embrace the extraordinary opportunities in the upcoming transportation revolution."
Masayoshi Son, chairman and chief executive of SoftBank, described Grab as "a tremendously exciting company in a dynamic and promising region.
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