The University of Central Lancashire organised the event as part of its Stressed Out Students (SOS)campaign, partnering with a local guide dog charity.
Students reserved time in advance and were given 10 to 15 minutes to play with the dogs in exchange for 1.50 pounds.
A total of 320 students visited the puppy room with a waiting list of people on standby, and each donated 1.50 pounds to the charity, according to the varsity press release.
"I have an exam and I was feeling stressed about it. Taking time out in the puppy room was definitely relaxing and allowed me to switch off for a bit. It was also great to learn more about how guide dogs are trained and work," said Andrew Ball, a first year sports therapy student who participated in the Thursday's event.
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"The event is a great opportunity for the students to meet our puppies and working guide dogs; helping them gain a real insight," said Guide Dogs Community Fundraiser Siobhan Cullen.
Colleges and universities across the US have therapy dogs for stressed students. Students report feeling more relaxed when they play with the dogs and are reminded of their pets back at home.