"Today I stand before the court to ask for the protection of the basic human rights of the Ukrainian people," Kiev's deputy foreign minister Olena Zerkal told the International Court of Justice in The Hague.
"Thousands of innocent Ukrainians have already suffered deadly attacks," she said.
Zerkal later told journalists on the steps outside the Peace Palace courthouse that "Ukraine will win this case because we are on the right side."
Nearly three years of conflict have claimed about 10,000 lives in eastern Ukraine -- and led to Russia's seizure of Ukraine's southern peninsula of Crimea in 2014 -- pushing ties between Moscow and the West to their lowest point since the Cold War.
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Zerkal told the court that Kiev was only seeking "a measure of stability and calm in an unpredictable and dangerous situation."
Ukraine is asking the ICJ to impose emergency measures ordering Russia to stop its alleged funnelling of money, weapons and personnel into the east, and to halt what it called "discrimination" against minorities in Russian-occupied Crimea.
Moscow has long denied arming the rebels and has said the case is motivated only "by political interests".
It also says Kiev has "shown a lack of will to hold a concrete dialogue."
Moscow has denied any involvement in the MH17 disaster and instead points the finger at Kiev.
Ukraine lodged its case at the ICJ against its former Soviet master in mid-January, saying it had protested for several years against Moscow's alleged financing of separatist rebels battling Ukrainian government forces.
Ukraine "respectfully requests the court to adjudge and declare that the Russian Federation bears international responsibility by virtue of its sponsorship of terrorism... for the acts of terrorism committed by its proxies in Ukraine," it said in papers before the court.
Ukraine was now facing a "human rights emergency," said Harold Hongju Koh, another of Kiev's representatives, adding that "in Crimea, Russia must cease its campaign of cultural erasure.
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