The local currency clawed back 60 kopeks to trade at 11.9 hryvnias to the dollar on the main interbank exchange.
The strong move came in response to the National Bank of Ukraine's announcement yesterday that it was raising its main interest rate to 9.5 from 6.5 per cent.
The bank also upped the overnight rate at which it provides emergency funding to other lenders by 700 basis points to 14.5 per cent.
The drop is explained in part by the National Bank of Ukraine's decision to stop propping up the currency in order to meet one of the main conditions set by the International Monetary Fund for its release of urgent economic aid.
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The Fund on March 28 announced the broad outlines of a USD 14-USD 18-billion package that could be disbursed over two years should Ukraine pursue painful and unpopular reforms.
But the Ukrainian bank is also trying to fight inflation that is expected to reach a rate of about 15 per cent this year.
Uncertainties linked to separatist tensions gripping the ex-Soviet country's Russian-speaking southeast is adding to investor worries and contributing to further currency falls.
Some economist said the bank's decision highlights the problems Ukraine's economy is facing despite the promise of Western aid.
"At the same time, tighter monetary policy will add to the pressures facing an economy that was already expected to contact by three per cent this year as a result of the political crisis and the austerity measures demanded by the IMF/EU.