"On this International Day against Nuclear Tests, I call on the world to summon a sense of solidarity commensurate with the urgent need to end the dangerous impasse on this issue," the Secretary General said in his message marking the International Day against Nuclear Tests.
He lamented that since its adoption 20 years ago by the General Assembly, the multilateral treaty has yet to enter into force.
"Given the catastrophic risks posed by nuclear weapons to our collective human and environmental security - even our very existence - we must reject this stalemate," he said.
In previous years, the UN chief had specifically asked India, Pakistan, China and the US to ratify the CTBT, saying that the entry-into-force of the Treaty is an "essential building block".
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Of these, China, North Korea, Egypt, India, Iran, Israel, Pakistan and the United States, have yet to ratify it.
Urging Member States to "act now", Ban said those States whose ratification is required to bring the Treaty into force should not wait for others.
"Even one ratification can act as a circuit breaker. All States that have not done so should sign and ratify because every ratification strengthens the norm of universality and shines a harsher spotlight on the countries that fail to act," he said.
He said a prohibition on all nuclear testing will end a "poisonous legacy", adding that it will boost momentum for other disarmament measures by showing that multilateral cooperation is possible, and it will build confidence for other regional security measures, including a Middle East zone free of nuclear weapons and all other weapons of mass destruction.
In 2009, the UN General Assembly had declared August 29 the "International Day against Nuclear Tests".
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