Guterres said the use of chemical weapons in Syria's nearly seven-year war "seriously challenges the global taboo against these weapons of mass destruction."
"If the use of chemical weapons in Syria is once again determined, the international community needs to find an appropriate way to identify those responsible and hold them to account," he told a council meeting on non-proliferation.
Russia used its veto power twice in November to block the renewal of a UN investigative panel tasked with identifying those responsible for chemical attacks in Syria.
Russia rejected the report's findings, saying the investigation was flawed because the experts did not travel to Khan Sheikhun and relied on witnesses accused of ties to the opposition of President Bashar al-Assad.
More From This Section
The Joint Investigative Mechanism (JIM), set up by Russia and the United States in 2015, shut down in November but Western powers have kept up efforts to restore some sort of mechanism for accountability.
US Ambassador Nikki Haley this month sent a letter to Guterres that contained a US rebuttal of Russia's objections to the gas attacks investigations in Syria.
Russia's arguments "are misleading, unprofessional, inconsistent, and at times, completely false," said the US assessment, seen by AFP.
"They appear to represent an intentional effort to sow confusion and to protect the Assad regime's continued use of chemical weapons," said the document that was sent to the council on January 10.
The Organisation for the Prohibition of Chemical Weapons (OPCW) continues to present to the council reports from its fact-finding missions in Syria.