The UN children's agency said it has documented a number of cases in which IS fighters killed the children of families trying to escape from neighbourhoods controlled by the militants.
"They are using children as a weapon of war to prevent people from fleeing," said UNICEF's Iraq representative, Peter Hawkins. "This just highlights how indiscriminate and catastrophic this war is."
Iraqi troops are slowly clearing the last pockets of IS fighters from Mosul's old city in an operation launched earlier this week. But an estimated 100,000 civilians packed into the dense terrain have slowed progress.
In the past six months alone, violence in Iraq has killed 152 children and injured 255, it said. In addition, the agency said, more than 1 million children have had their educations put on hold by either militant rule or displacement.
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Children have also been forced to take part in violence. UNICEF said at least 231 children under the age of 18 were recruited by IS and other armed groups.
"The country's future security and economic strength is determined by what is happening to its children today," Hawkins said.
Late yesterday, the al-Nuri Mosque and iconic leaning minaret in the Old City were destroyed. Iraqi and coalition officials said IS fighters blew up the landmarks. IS released a statement blaming a US airstrike.
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