The Hoh Xil nature reserve, located on the Qinghai-Tibet Plateau, is home to over 200 animal species. More than 20 of them are state-protected, including the Tibetan antelope.
It is China's largest world natural heritage site, covering an area of 45,000 square kilometres.
"Just inscribed as @UNESCO #WorldHeritage Site: Qinghai Hoh Xil, #China," the UN's cultural arm tweeted during its World Heritage Committee meeting in Krakow.
A handful of pro-Tibet activists protested earlier this week while the International Campaign for Tibet (ICT) advocacy group warned that giving Hoh Xil heritage status could have consequences for Tibet.
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"The inscription of Hoh Xil without further detailed assessment would effectively signify endorsement from the international cultural heritage body of China's ambitious policies including the displacement of Tibetan nomads from their land," the group added.
China has invested billions of dollars into resettling Tibetan herders, who have for centuries led a nomadic life, moving regularly to seek fresh grazing for their animals.
But while some Tibetans welcome the changes, many worry about the disappearance of a lifestyle that has endured for hundreds of years, and see the resettlements as part of a broader erosion of Tibetan culture in China.
China's representative insisted at the UNESCO meeting today that Beijing would never engage in forced resettlement on the Hoh Xil reserve, according to the Polish news agency PAP.