The ruling BJP-Sena also demanded probe in the multi-crore Annabhau Sathe Corporation scam and arrest of middlemen in the Telgi case.
The House saw disruptions twice before being adjourned for the day as Speaker Haribhau Bagde rushed through the day's agenda amid pandemonium.
The Treasury and Opposition members displayed placards and shouted slogans resulting in ruckus.
Earlier, Opposition members gathered on the stairs of the Vidhan Bhavan building and staged a sit-in protest demanding resignation of Education Minister Vinod Tawde over his alleged fake degree, Women and Child Welfare minister Pankaja Munde in the 'chikki' scam as well as Water Supply Minister Babanrao Lonikar and Minister of State for Home, Ranjit Patil against whom allegations have been made.
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To counter the Opposition charge, ruling BJP-Sena legislators demanded action against NCP MLA Ramesh Kadam over allegations of siphoning-off funds of Annabhau Sathe Corporation and demanded his arrest.
The ruling party members also displayed placards about arresting middlemen of Abdul Karim Telgi, the main accused in the multi-crore fake stamp paper case following which the House had to be adjourned for 20 minutes.
Later, Vikhe Patil and NCP leader Jayant Patil persisted with the demand for resignation of ministers and criticised Chief Minister Devendra Fadnavis for giving them a clean chit without probe.
Rejecting the Opposition demand, Fadnavis said his government was open to probe in all allegations.
"If you feel that we will bow down to your demand, you are mistaken," he said.