Deputy Leader of the Opposition and DMDK MLA, R Mohanraj without naming AIADMK chief Jayalalithaa made an implied reference to her, which was strongly opposed by the treasury benches.
Ruling party members led by Chief Minister O Panneerselvam urged Speaker P Dhanapal to initiate appropriate action against Mohanraj.
Mohanraj's remark came after an AIADMK MLA made an indirect reference to DMDK chief Vijayakanth, which was considered disparaging by his party members.
DMDK MLAs led by party Whip V C Chandrakumar rushed towards the Speaker's podium to protest his eviction.
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DMDK MLAs were evicted forcibly by the marshals and some of them were attacked in the corridor by the MLAs.
Speaker P Dhanapal called the incident a historic low in the annals of the Tamil Nadu Assembly.
"The DMDK MLAs created an unsafe situation for the Speaker, defied his orders, intimidated and attacked the marshals. Chandrakumar and others have repeatedly created ruckus and brought disrepute to the House," Viswanathan said.
DMDK MLAs C H Sekar, K Thinakaran and others attacked and injured the Marshals outside the House (at the corridor) and action will be taken against them, he told the House.