White House spokesman Ned Price says the money will help pay for food, clean drinking water and improved sanitation, among other necessities.
Nearly 13 million Yemenis are facing food shortages that are expected to worsen as the conflict continues.
Yesterday's announcement brings the total US contribution for humanitarian needs in Yemen to USD 170 million for the budget year ending September 30.
Yemen's conflict pits various forces against the Houthi rebels, who are allied with security forces loyal to former President Ali Abdullah Saleh.