"We urge Singapore to ensure that freedom of expression is protected in accordance with its international obligations and commitments," State Department spokeswoman Jen Psaki said.
"We are concerned... To see Singapore applying press restrictions to the online world."
The surprise regulations came into force on June 1 requiring news websites -- including one operated by US-based Yahoo! -- to obtain licenses from the city-state's official media regulator.
Last month, Singaporean bloggers blacked out their homepages for 24 hours to protest the measure which they say will muzzle freedom of expression.
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Singapore's media regulator, the Media Development Authority (MDA), and government leaders had sought to allay fears the rules were aimed at the feisty blogging community, pointing out that blogs were not considered news portals.
But websites granted a licence will have to remove "prohibited content" such as articles that undermine "racial or religious harmony" within 24 hours of being notified by the authority.
Bloggers participating in the Internet blackout insisted the law's broad reach was indicative of the government's intentions to require blogs to seek licensing in the future as well.