"Six US Air Force F-16 Fighting Falcons deploy to Incirlik Air Base, Turkey, to support the fight against ISIL," it said in a tweet, referring to a variant name for the jihadist group.
The United States has already been using drones from Incirlik to strike IS targets in Syria, supporting the Turkish air campaign against the militants.
"At approximately 0900 (GMT), the US Air Force deployed a small detachment of six F-16 Fighting Falcons, support equipment and approximately 300 personnel to Incirlik Air Base, Turkey, in support of Operation Inherent Resolve," US European Command said in a press release.
Turkey, a member of the international coalition led by its NATO ally Washington, stepped up action against jihadists after a deadly bombing in July in a border town.
According to media reports some 30 US fighters are due to arrive in the coming days to take part in the operation.