The seven were killed yesterday by unidentified assailants in a raid on their base in Sarmin, nine kilometers east of the city of Idlib.
The town is controlled by the Hayat Tahrir al-Sham jihadist alliance, which denounced what it called an "ugly crime."
State Department spokeswoman Heather Nauert extended condolences to the families of the victims.
"We are saddened and horrified to hear about the brutal murders of seven Syrian Civil Defense Members, more widely known as the White Helmets," she said.
The White Helmets emerged in 2013, working to rescue civilians in rebel-held areas.
They have since gained international renown for their daring rescues, often filmed and circulated on social media, and were nominated for the 2016 Nobel Peace Prize.
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