Russia pointed an accusatory finger at the coalition a day after Friday's incident, in which local officials said women at a shrine in the town of Daquq were killed by an air strike.
The coalition "has determined definitively that we did not conduct the airstrike w/reported civilian casualties in Daquq," spokesman Colonel John Dorrian said on social media.
The local council chief and medics in Daquq, which lies south of Kirkuk and about 200 kilometres north of Baghdad, said the deadly incident was caused by an air raid.
Neither have made any comment yet but the office of Iraqi Prime Minister Haider al-Abadi said it ordered an investigation into the incident.
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"The results will be announced as soon as it is completed," a statement said.
Turkish jets have also routinely conducted air strikes in Iraq but they usually target Kurdish rebel positions in areas far removed from Daquq.
Recent incidents and discoveries in workshops used by the Islamic State group suggest the jihadists have been trying to develop weaponised drones.
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