Of that number, 157 live in the 50 US states and Washington, while the other 122 live in Puerto Rico, the Centers for Disease Control and Prevention said.
All have "laboratory evidence of possible Zika virus infection," the CDC said.
The new figure appears to be a dramatic jump from the 110 pregnant women with confirmed Zika cases that the CDC reported as of May 11, but officials say the figures are not comparable, as a new reporting system is being used.
In Brazil, 1,271 babies have been born with unusually small heads and deformed brains since the outbreak of Zika began there last year.
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The virus, which usually causes only mild, flu-like symptoms but can cause the rare but serious neurological disorder Guillain-Barre Syndrome, is mainly spread by two species of Aedes mosquito but has also been shown to transmit through sexual contact.
The CDC is now using two separate registries to track pregnant women residing in the United States and all territories, as Puerto Rico is keeping separate records.
"We've learned a lot in the last four months," said Margaret Honein, a top official at the CDC's National Center for Birth Defects and Developmental Disabilities.
Scientists now know of cases of infants born with microcephaly whose test results suggest a Zika infection, but whose mothers did not remember having any symptoms, she said.
The new surveillance systems "cast a broad net to ensure we are monitoring all pregnant women who may be at risk for poor outcome associated with Zika," Honein said.
At least five women contracted Zika without leaving the continental US, but had sexual relations with someone who had, Honein said.