Some of the graphic images of the chemical attack have been shown in the videos, which come days ahead of the debate and vote in the Senate on the resolution to authorise US President Barack Obama to launch "limited" military strikes against the Assad regime.
The videos, first played to the members of the Senate Intelligence Committee on Thursday were first leaked to CNN, which many media outlets said is part of an effort to build public opinion on the Syria attack.
Some of the latest opinion polls have showed that the appetite for another war in the US is very low.
The Senate Intelligence committee, in a statement, said that the 13 videos were compiled by the US Open Source Center from footage taken in the Damascus suburbs on August 21, the day of the alleged attack.
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"All of the videos were posted on YouTube by pro-Syrian opposition users," the committee said.
The Senate Intelligence Committee Chairwoman Dianne Feinstein said early this week that she asked for the DVD to provide "specific instances of evidence, largely victims, and what we see means, what pinpointed eyes mean, what the convulsions mean, a number of aspects."
"They took 170 videos and siphoned them down to 13, which have very probative points in them, and dispositive, with respect to the use of chemical weapons," she said.
The US Senate is expected to vote on it early this week, even as Secretary of State John Kerry is travelling in Europe garnering international support.