The two countries are permanent UN Security Council members and among the nine states confirmed or believed to possess nuclear weapns, but shunned gatherings held in Norway last year and Mexico in 2014.
Today's meeting featured some 800 delegates from close to 160 countries discussing the world's 16,300 nuclear weapons.
Other weapons holders present in Vienna were Pakistan and India, but Russia, France and China did not send official delegations, although a Chinese think-tank close to the country's government was present, organisers said.
The two-day meeting focused on the potential short- and long-term humanitarian consequences of a nuclear explosion, the impacts of nuclear testing and the risks of an accidental atomic blast.
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It included Setsuko Thurlow, 82, a wheelchair-bound survivor from the US atomic bombing of Hiroshima, Japan in 1945, who made an impassioned speech warning against the "ultimate evil" of nuclear weapons.
Nuclear weapons have only been used in war twice -- in Nagasaki and Hiroshima -- but there were more than 2,000 test explosions between 1946 and when the practice largely halted in the 1990s.
"As long as nuclear weapons exist, the risk of their use -- deliberately or inadvertently -- remains real. Such a scenario, more than any other human action, has the potential of ending life on this planet as we know it," said Sebastian Kurz, foreign minister of hosts Austria.
Ahead of the conference a group of 118 statesmen and women from 46 countries issued a joint statement warning that the risks posed by nuclear weapons "are under-estimated or insufficiently understood by world leaders".