Additional Sessions Judge Raj Kapoor denied the relief to accused Vivek Srivastava, who was working in the hotel when the crime was allegedly committed.
The court rejected the plea, moved by advocate Sandeep Kapur, saying that the allegations levelled by the US national against the accused were grave and his case was not fit for bail.
Srivastava, along with three other accused Aniruddha Singh, Omprakash and Maqsood, is at present lodged in judicial custody.
The US national arrived here in December last year to join the probe and recorded her statement before a judicial magistrate where she reiterated that she was gangraped by five persons in the five-star city hotel.
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Four accused were arrested after the Delhi Commission for Women (DCW) issued a notice to the police asking them why the Rape Crisis Cell of the panel was not informed when the victim's statement was recorded.
Earlier, the complainant had said she was "not satisfied" with the probe and was ready to come to India to identify the accused.
She had also stated that the accused had made a video of the act and threatened to make it public if she reported the matter to anyone. She had also told the police that the accused had claimed to have sexually assaulted a couple of other female tourists as well.
A team of psychologists and psychiatrists had assisted the woman in recalling the details of the incident.