Metropolitan Magistrate Harvinder Singh sent the accused to Tihar Jail after Delhi Police moved an application seeking their judicial custody.
Yesterday, the police had arrested a tour guide, a driver, a cleaner and a hotel staff in connection with the alleged gangrape of the woman in April this year.
The US national arrived here a few days ago to join the probe and recorded her statement in front of a judicial magistrate where she reiterated that she was gangraped by five persons in a five-star hotel here.
Earlier, the complainant had said she was not "satisfied" with the probe and was ready to come to India to identify the accused.
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The woman had alleged that she was raped by the men for two days and also threatened with dire consequences if she reported the matter to anybody.
She had also stated that the accused had made a video of the act and threatened to make it public if she reported the matter to anyone. She had also told the police that the accused had claimed to have sexually assaulted a couple of other female tourists as well.