Vaiko, Tamil Desiya Iyakkam (Tamil Nationalist Movement) leader P Nedumaran and around 400 people owing allegiance to about 20-odd pro-Tamil organisations were arrested, police said.
The protestors also demanded that India support a US-sponsored motion against Sri Lanka in the UN Human Rights Council, and raised slogans against island country President Mahinda Rajapakse.
They were also demanding closure of the mission in the city.
Political parties in the state have been pressing for action against the Sri Lankan government for the alleged war crimes committed by its army during the last phase of the conflict with rebel LTTE.
DMK, a key UPA ally, has been insisting that India sould support the US-sponsored Sri Lanka-specific resolution in the ongoing UNHRC meet at Geneva.
The chorus in the state for India joining hands with the US grew stronger following the alleged cold-blooded killing of slain LTTE chief V Prabakaran's 12 year-old son Balachandran.