Known for her fearless advocacy of all human beings, 41-year-old Gupta will replace Wade Henderson who is stepping down after more than two decades.
Gupta, who will also lead its sister organisation 'The Leadership Conference Education Fund', will begin her new role on June 1.
"At a time when our nation's ideals and progress are being threatened in such fundamental ways, The Leadership Conference is a vital nerve center of the broad swath of civil and human rights organisations that are fighting for justice, fairness, and equality around the country," Gupta said.
Henderson, who will step down after 21 years, said leaders had the responsibility to cultivate, encourage and make paths for the next generation to lead and to thrive.
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"Her fearless advocacy for the rights of all Americans, while at the helm of the Civil Rights Division, proves that she will be able to lead the important coalition of the Leadership Conference member organisations," Holder said.
As head of the civil rights division, Gupta oversaw a wide range of criminal and civil enforcement efforts to ensure equal justice and protect equal opportunity for all during one of the most consequential periods for the division.
She regularly engaged with a broad range of stakeholders in the course of this work.
Prior to joining the Justice Department, Gupta served as Deputy Legal Director and the Director of the Center for Justice at the American Civil Liberties Union (ACLU).
She joined the ACLU in 2006 as a staff attorney, where she subsequently secured a landmark settlement on behalf of immigrant children from around the world detained in a privately-run prison in Texas that ultimately led to the end of "family detention" at the facility.
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