Daniel Tinoco, 24, was killed Monday night in the western city where nationwide protests against the leftist government of President Nicolas Maduro first ignited February 4.
Tinoco died from a gunshot wound to the chest, opposition Mayor Daniel Ceballos told AFP yesterday.
Ceballos said Tinoco had no vital signs upon arrival at a clinic.
"Two other people who were shot -- one in the shoulder and the other in the abdomen -- were operated on in the early morning and are in a stable condition," he said.
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The mayor said he "presumed" that the attackers were part of "armed groups that support the government."
The anti-government demonstrations that have rocked Venezuela for more than a month have been fueled by rising discontent over deteriorating living conditions and police crackdowns in the oil-rich OPEC nation.
Maduro's socialist government has outraged many particularly with shortages of basic goods like toilet paper and food, and amid spiraling violent crime.
Disgruntled students and government critics announced fresh protests set for today in the Caracas neighborhood of Libertador.
Since protests began, opposition leaders and government officials have blamed each other for the deadly violence.
On Monday, Maduro reported that authorities are investigating the fatal shooting of a Chilean citizen over the weekend during a protest in Venezuela's western city of Merida.
Protests have left more than 300 people wounded and have spread to cities such as Caracas, Merida, Valencia and Maracay.