Foreign Minister Samuel Moncada said on state television that the election of members of a constitutional assembly will take place as planned on July 30. He said President Nicolas Maduro has asked him to reconsider the country's diplomatic relations with the US.
"The constitutional assembly is happening," Moncada said, adding that Venezuela is "conducting a deep review of relations with the US government because we don't accept humiliation from anyone."
The US is a major market for the oil exports that drive Venezuela's economy. Trump has imposed travel bans and has frozen the assets of high-ranking officials in recent weeks, but refrained from broad sanctions against the country that could deepen its economic crisis.
Venezuela's opposition called Monday for a 24-hour nationwide strike to pressure Maduro to drop his plans to rewrite the constitution. The opposition said that more than 7.5 million people voted against the constitutional assembly at unofficial ballot boxes set up nationwide and in expatriate communities Sunday.
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Canada, Mexico, Brazil, Germany, Argentina, Colombia and the European Union have also come out against the effort.
The opposition said it would launch a plan it called "zero hour" on Wednesday that includes an agreement to form an alternate government and create 2,000 local committees that would function as street-level support for the opposition.
That would be followed Thursday by a nationwide strike, which could bring much of Venezuela's already sputtering economy to a standstill. Venezuela's largest chamber of commerce told The Associated Press that its members would not punish employees for participating in the strike.
Those nominations would not give the opposition a supreme court majority but are almost certain to be rejected by the current court and the executive branch, making them a largely symbolic tactic to increase pressure on Maduro.
More than three months of street protests have left at least 93 people dead and 1,500 wounded. More than 500 protesters and government opponents have been jailed.
Opponents of Venezuela's government blame it for turning one of the region's most prosperous countries into an economic basket case with a shrinking economy, soaring inflation and widespread shortages. The government blames the crisis on an economic war waged by its opponents and outside backers.