Dee passed away peacefully from age-related causes on Wednesday night, said The Hollywood Reporter.
Dee had an impressive stage career, including a highly praised performance in 'Purlie Victorious' (1963). Also on stage, Dee was notable as the proud working mother Ruth in 'A Raisin in the Sun' (1961).
Dee won an Obie Award in 1971 for her portrayal of Lena in Athol Fugard's 'Boseman and Lena' (1970) and a Drama Desk Award for 'Wedding Band' (1974). She was nominated for an Academy Award for her role in Ridley Scott's 2007 drama 'American Gangster'.
Along with Davis, she was honored with the Screen Actors Guild's Life Achievement Award in 2001. They were the second couple to receive that distinction, with Paul Newman and Joanne Woodward honored in 1995.
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Dee was the first black actress to play a leading role in the American Shakespeare Festival, when she acted in 'King Lear' (1965). She later triumphed in 'The Taming of the Shrew' (1965) on Broadway.
Dee and Davis conceived the acclaimed PBS series 'With Ossie & Ruby' (1981). The couple co-hosted, performed and co-produced 26 half-hour programs. She co-produced 'The Ossie Davis and Rudy Dee Story Hour'.