Looking at samples from the world's largest natural asphalt lake, they found active microbes in droplets as small as a microlitre, which is about 1/50th of a drop of water.
"We saw a huge diversity of bacteria and archaea," said Dirk Schulze-Makuch, a professor in Washington State University's School of the Environment.
"That's why we speak of an 'ecosystem,' because we have so much diversity in the water droplets," said Schulze-Makuch.
The researchers found that the microbes were actively degrading oil in the asphalt, suggesting a similar phenomenon could be used to clean up oil spills.
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"But this shows astrobiology has also great environmental applications, because of the biodegradation of oil compounds," he said.
Schulze-Makuch and his colleagues in 2011 found that the 100-acre Pitch Lake, on the Caribbean island of Trinidad, was teeming with microbial life, which is also thought to increase the likelihood of life on Titan.
The research adds a new, microscopic level of detail to how life can exist in such a harsh environment.
Analysing the droplets' isotopic signatures and salt content, the researchers determined that they were not coming from rain or groundwater, but ancient sea water or a brine deep underground.
The research was published in the journal Science.