Two women, both sisters, and the daughter of one of them were going on a scooty from Vijayawada to Avanigadda when the tractor hit their vehicle near Veluvolu village at around 7.30 am, Challapalli circle inspector V Janardhana Rao said.
In the mishap, Ankamma (47) died on the spot, while her niece Sivaleela (15) succumbed to her injuries on way to hospital, he said.
Sivaleela's mother, who also sustained injuries in the mishap, was admitted to Avanigadda Government Hospital where her condition was reported to be stable, Rao said.