The study into lethal wounds found on the skull also offers evidence of the earliest funerary practices in the archaeological record, researchers said.
The research, conducted by an international team of collaborators including Binghamton University anthropologist Rolf Quam was carried out at the archaeological site of the Sima de los Huesos in northern Spain.
The site is located deep within an underground cave system and contains the skeletal remains of at least 28 individuals that date to around 430,000 years ago, during the Middle Pleistocene time period.
A nearly complete skull, Cranium 17, from the Sima de los Huesos is comprised of 52 cranial fragments recovered during excavations at the site over the last 20 years.
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This skull shows two penetrating lesions on the frontal bone, above the left eye.
"Evidence for interpersonal violence in the human fossil record is relatively scarce, and this would appear to represent the coldest cold case on record," said Quam.
Relying on modern forensic techniques, such as contour and trajectory analysis of the traumas, the authors of the study showed that both fractures were likely produced by two separate impacts by the same object, with slightly different trajectories around the time of the individual's death.
Rather, the type of fracture, their location and that they appear to have been produced by two blows with the same object lead the authors to interpret them as the result of an act of lethal interpersonal aggression - or what may constitute the earliest case of murder in human history.
Furthermore, if this individual was already dead, other humans likely carried them to the top of the vertical shaft.
The authors suggest that humans were likely responsible for the accumulation of bodies in the Sima de los Huesos, which supports the idea that this site represents early evidence of funerary behaviour.
The study was published in the journal PLoS ONE.