The blue and white LEGO boat measures 12.035 metres in length.
The new Guinness World Records title was confirmed at an event organised to celebrate the 150th anniversary of the shipping and transport services company DFDS A/S in Copenhagen.
It took 900 person hours to build and thousands of DFDS employees from across the world got on board, assisted in the challenge by a few LEGO building professionals, Guinness said.
The Jubilee Seaways will be taken around Europe in a container truck to give people the chance to admire the record-breaking creation.