Younis, 38, informed chief selector Inzamam-ul-Haq that he still required 8-10 days more to make a complete recovery from the bout of dengue that he had recently suffered and for which he had to be hospitalised.
"Yes, Younis has ruled himself out of the first Test and has asked for another 8 to 10 days to make a complete recovery," a Pakistan Cricket Board official said.
Younis was last month admitted to a private hospital after suffering from high fever and fatigue and was only released after a week.
The first Test between Pakistan and West Indies will be a day/night match with the pink ball, only second such match in Test history. The first was played between Australia vs New Zealand in Adelaide last year.
Young batsman Babar Azam, who reeled out three successive centuries against the West Indies in the three-match ODI series, is seen as a likely replacement for Younis in the first Test.
Younis will rue missing the Test against the depleted and demoralised West Indies as he requires another 544 runs to become the first Pakistani to complete 10,000 Test runs.
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