Yuvraj was fit to play tomorrow's game, according to team coach Allan Donald, but Pune will miss West Indian all-rounder Marlon Samuels, who has picked up a groin injury.
The Warriors have lost two matches on the trot thanks to dismal batting efforts.
On the other hand, the Royals go into their second away game after recording a clinically executed and morale-boosting 19-run victory over defending champions Kolkata Knight Riders at home, their second win in as many matches.
Yuvraj pulled out of Pune's first home game on April 7 against Kings XI Punjab at the eleventh hour, after flopping in their campaign opener in Hyderabad against new outfit Sun Risers, complaining of a sore back.
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How and when did he sustain the injury is not known and in his absence, the Warriors were restricted to a paltry 99 for nine, which was easily overhauled by Kings XI.
The Warriors would be happy to get such a key player back after suffering successive defeats.
Pune have been done in by the poor form of their top batsmen who need to contribute significantly if are to outdo the high-flying Royals who shot to the top of the table after stunning KKR.