Czech Republic winger Vaclav Pilar scored an injury-time winner for his side to beat Netherlands, who suffered their second straight defeat since Guus Hiddink took charge of the Dutch team.
Borek Dockal had given the hosts the lead in the Euro 2016 qualifier in Prague before Netherlands' Stefan de Vrij equalised. But Daryl Janmaat's mistake allowed Pilar to seal the points as Hiddink became the first Dutch national manager to lose his first two games since his previous reign in 1995.
Meanwhile, Italy beat Norway 2-0, thanks to goals by Simone Zaza and Leonardo Bonucci, to extend Antonio Conte's winning start since taking over following Cesare Prandelli's resignation, The BBC reported.
Also in Group H, Real Madrid's former Tottenham Hotspur midfielder Luka Modric and Andrej Kramaric scored as Croatia overcame 10-man Malta 2-0, while Bulgaria won 2-1 away to Azerbaijan.
Wales' Group B rivals Cyprus came from behind to register a shock win 2-1 away to Bosnia-Hercegovina. Vedad Ibisevic put Bosnia ahead, but Demetris Christofi equalised and then scored again in the 73rd minute.
In Group A Iceland ran out 3-0 winners at home to 10-man Turkey. Dadi Bodvarsson put them ahead before Turkey's Omer Toprak was sent off for two yellow cards. Swansea City's Gylfi Sigurdsson and Kolbeinn Sigthorsson scored two goals in one minute to round off the win, the report added.