Dutch international Leroy Fer has suffered a hamstring injury during training here Tuesday.
According to the Dutch football association KNVB, examination in the forthcoming days will reveal the extent of the injury, reports Xinhua.
After feeling pain in his left thigh, he limped off training at the Flamengo training accommodation.
Fer debuted at the World Cup Monday against Chile (2-0) as a second half substitute.
Two minutes after his entrance in the 75th minute he opened the scoring with a firm header.
Meanwhile, Bruno Martins Indi returned to group training Tuesday.
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He seems to have recovered from the concussion he suffered in the second match against Australia (3-2).
Dutch coach Louis van Gaal had prepared a light post-match training.
The Netherlands meet Mexico in FortalezaAin the last-16 of the World Cup.