The couple, who tied the knot in 2001, already have an 11-year-old son named Aarav.
"Thank you... Thank you very much. She is fine," an elated Akshay told reporters when asked about his wife's second pregnancy.
Twinkle is likely to make her appearance during filmmaker Karan Johar's 40th birthday party on May 25.
Post her marriage, Twinkle, 37, quit acting and since then has been working as an interior designer.
