Australia captain Michael Clarke will miss his side's tour match against Sussex this week, while rookie off-spinner Ashton Turner is set to make his first-class debut for the struggling tourists at Hove.
Clarke is sidelined amid reports that a long-standing back injury flared up after Australia's crushing second Test defeat against England at Lord's.
Ed Cowan will skipper the team in Clarke's absence, with vice-captain Brad Haddin, openers Shane Watson and Chris Rogers plus pace bowlers Peter Siddle and Ryan Harris all rested.
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The 20-year-old has taken 18 wickets at an average of 40.78 for the Sussex Premier League club this season.
Turner, a member of the Australia Under-19 team that finished runners-up to India in last year's World Cup, was one of six Australian Institute Of Sport scholars to be awarded a summer placement in England.
His call-up means Australia will field three spinners against Sussex as Ashton Agar and Nathan Lyon were already included.
With Australia in crisis after falling 2-0 behind in the five-Test series, their hopes of regaining the Ashes suffered a further blow on Monday when pace bowler James Pattinson was ruled out for the rest of the series with a stress fracture of his lower back.
The tour match against Sussex, which starts on Friday, now serves as an opportunity for Mitchell Starc, Jackson Bird and James Faulkner to win a place in the Australia attack for the third Test at Old Trafford, which gets underway next week.