The pop star is the latest musician to join Winfrey on her OWN show 'Oprah's Next Chapter' after Rihanna and Fergie.
The episode will go backstage at a Bieber Believe tour date as well as include a sit-down interview, the Hollywood Reporter said.
"Me and the Bieber just finished our interview," Winfrey wrote on Facebook on Wednesday, sharing a picture of herself with the teen singer. "Good stuff!," she added.
Bieber has been busy with his "Believe" world tour to promote his album of the same name, extends through April.