American car customiser George Barris transformed the banger into the sleek black vehicle that starred in the legendary film and TV series in 1966, reported Daily Mail.
Now Barrios, 87, who has owned it ever since, has decided to sell the caped crusader's company car.
"I think it is the most valuable car in the world and the public really get a kick out of it when they see it. I designed and built the Batmobile and I have shown it all around the world but now I think it is time that someone else can enjoy it," Barris said.
It will be auctioned off by Barrett Jackson on January 19 at its sale in Scottsdale, Arizona with both auction house and owner tight-lipped on the car's reserve.
However, both parties expect the 19ft long piece of automotive history will sell for multiple millions.