Police officials said DMK MP Ritheesh also appeared before the District Police, and sought time for replying to their questions.
Police had asked Dayanidhi's wife Anushka if he had contacted her anytime, sources said.
Police had also issued summons to Anushka's father and other relatives.
The Deputy Mayor of Madurai Mannan had appeared before police for questioning yesterday, sources said.
Dayanidhi, for whom a special police team is on the lookout, and nine others are facing charges that companies owned by them mined sand and granite without permission.
Police have charged Dayanidhi and Nagarajan, partners in Olympus Granites Pvt Ltd, with encroaching on government land and taking away large quantity of granite illegally without quarrying in the permitted area.
Government cracked down on the firms after former Madurai Collector U Sahayam in a report, estimated the losses incurred from illegal granite mining at around Rs 16,000 crore.
The Madurai Bench of the Madras High Court had on September 25 rejected Dayanidhi's anticipatory bail plea in the case.
