Russia has the right to "protect its interests and Russian-speaking populations" in Ukraine, President Vladimir Putin has told his US counterpart Barack Obama.
Putin told Obama there was a "real threat weighing on the lives and the health of Russian citizens" in Ukraine, according to a Kremlin statement.
The Russian president accused the new government in Kiev of supporting "criminal actions by ultranationalists", the Kremlin said.
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The Kremlin statement said the conversation was instigated by the US side and focused on the "extraordinary situation in Kiev".
It came after Obama's national security team met yesterday to weigh policy options as Washington tried to keep pace with the fast escalating crisis in Ukraine and Crimea.