Opposition-controlled parts of Syria's battered northern city Aleppo came under total siege today, after government forces severed the last route out of the east.
An estimated 300,000 civilians live in rebel-held neighbourhoods of Syria's second city, the UN says, and there are fears that they could face starvation.
The rebels have failed to thwart a major Russian-backed army offensive around Aleppo, which has been devastated by Syria's five-year conflict.
Today, regime fighters descended on the Castello Road and fully cut it, said Rami Abdel Rahman, head of the Britain-based Syrian Observatory for Human Rights.
"The eastern neighbourhoods are now completely besieged," he told AFP.
The Observatory also reported that five civilians were killed today in regime air strikes on the rebel-held neighbourhood of Al-Maysar while 16 rebel fighters died in the regime advance on Aleppo.
The city is divided roughly between government control in the west and rebel control in the east.
The Castello Road had been used by rebels but also by shopkeepers bringing in produce for residents and by villagers visiting relatives in the city.
"Aleppo is now 100-percent besieged," one rebel from the Aleppo Revolutionaries group told AFP.
He said the army had reached the road and was setting up sandbag barriers.
Facebook pages run by Aleppo-based activists urged civilians to stay away from the route to avoid being arrested or wounded.
Sieges by both the regime and its opponents have had a devastating impact on other areas of Syria, including the town of Madaya where aid groups say dozens of people have died from starvation and malnutrition.
The United Nations says that nearly 600,000 people are living under siege in Syria, most of them surrounded by government forces.
Eastern Aleppo has not been designated by the UN as besieged, but residents complain of food shortages and shopkeepers have begun rationing their products.
A leading opposition group warned last week that hundreds of thousands of civilians in the city were at risk if the Castello Road was cut.
Anas al-Abdeh, head of the Istanbul-based opposition National Coalition, said his group feared "that if the Castello route is totally cut off, more than 300,000 civilians will starve".
Fighters loyal to President Bashar al-Assad have tried over the past two years to cut the strategic road as part of a campaign to retake all of Aleppo.
