The 'Wannabe' hitmakers were last year asked to appear at the show, which took place outside Buckingham Palace in London on Monday, but declined due to their heavy workloads.
But after viewing the musical extravaganza on television, singers Melanie Chisholm, Melanie Brown and Emma Bunton all took to Twitter to express their sadness at not being involved, reported Contactmusic.
Brown, who is currently based in Australia, wrote, "Makes me sooo (sic) sad spice girls wasn't part of the queens (sic) jubilee, break my heart big time, i was so up for it, watched some of it on tv, amazing!"
"Bit miffed I'm not down at Buckingham Palace," said Chisholm and Bunton re-tweeted several messages from fans, including one which reads, "Ok the spice girls should have rocked this show!!"
