Vijayamma, who is YSR Congress' honorary president, raised the issue while addressing roadside meetings at Rayachoty, Rajampeta and Kodur towns as part of a by-election campaign.
She demanded that transcripts of the conversation recorded in the chopper's black box be published.
The Pulivendula MLA had questioned the chopper crash investigation earlier in the campaign too.
Vijayamma also alleged that her son Jagan Mohan Reddy was being targeted by the government by being falsely implicated in corruption cases.
Her daughter, Sharmila, alleged that the CBI was a "puppet organisation" in the hands of the central government and had colluded with Congress and TDP to harass Jagan.
